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Characterization of antibodies specific for amyloid proteins
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health. (Gunilla Westermark)
2015 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Amyloidosis is a group of diseases caused by proteins that have lost their correct three-dimensional conformation and instead assemble into insoluble fibrils in various tissues and organs. Today, more than 30 different proteins that can give rise to amyloid fibrils have been identified. Each protein that assembles into fibrils causes a specific disease. For clinical diagnosis of amyloid, antibodies are one of the most important tools.

In this study, antibodies generated towards various amyloid-specific peptides were characterized and validated. This was assessed by immunohistochemistry, slot blot and SDS-PAGE and western blot. Congo red, an amyloid specific dye, was used for detection of amyloid.

Immunohistochemical staining and slot blot analysis indicated that each antiserum used in this study was amyloid-specific. Antigen retrieval can facilitate staining by the techniques ability to break cross-linkages caused by fixation in formaldehyde.

The results from the characterization of antisera in this study should be a great helpin clinical work on amyloid, and ensure correct diagnosis.

Place, publisher, year, edition, pages
2015. , 31 p.
Keyword [en]
Amyloidosis, Congo red, Immunohistochemistry, Polarizing microscope, Slot blot
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-254597OAI: oai:DiVA.org:uu-254597DiVA: diva2:818980
Educational program
Biomedical Laboratory Science Programme
Available from: 2015-06-09 Created: 2015-06-09 Last updated: 2015-06-09Bibliographically approved

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