Evaluation of a standardized platelet concentration in samples from platelet concentrates measured over time with impedance aggregometry
Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Platelet transfusions can be necessary during treatment of patients with thrombocytopenia or impaired platelet function. Platelet function in platelet concentrates (PC) deteriorate with storage time. Studying swirling is often used to control the quality of PC’s before transfusion but the method has some disadvantages. Therefore other methods can be useful, for example impedance aggregometry (IA, Multiplate® Analyzer) to measure platelet function.
In this study the change in platelet function over time was examined in buffy coat and apheresis platelets with IA where aggregation had been induced with adenosine diphosphate (ADP) and collagen. PC’s were tested on day 1, 4 and 7 after donation. One of the main aims of this study was to evaluate if dilution to a standardized platelet concentration (800x109 platelets/L) for IA of PC’s could be used, since platelet concentration has been shown to influence aggregation. The effect of pathogen inactivation (INTERCEPT) on platelet function and the importance of fibrinogen for aggregation were also studied.
The dilution of platelet samples reduced the range of measured values and was suitable to use with collagen but not ADP. The platelet function decreased significantly over time with both agonists. There was a significant difference between pathogen inactivated and gamma irradiated PC’s with collagen activation on day 1. Fibrinogen was shown to be of importance for platelet aggregation, but other factors in plasma seem to be necessary too.
In conclusion, IA is a suitable method for following change in aggregability over time in PC’s and sample dilution reduced variation in results.
Place, publisher, year, edition, pages
2015. , 22 p.
Platelet function, apheresis, buffy coat, adenosine diphosphate, collagen
Biomedical Laboratory Science/Technology
IdentifiersURN: urn:nbn:se:uu:diva-254606OAI: oai:DiVA.org:uu-254606DiVA: diva2:818999
Biomedical Laboratory Science Programme