uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Newly Formulated Idebenone and Inflammatory Pain
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2015 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Newly Formulated Idebenone and Inflammatory Pain

Daniel Gallo

Carolina Muscoli, Department of Pharmacotherapy, 15 ECTS credits. Margareta Hammarlund-Udenaes.

Introduction: MnSOD (endogenous antioxidant) plays a part in maintaining the levels of free radicals. An increase of these levels will lead to oxidative stress that can result in imune- and inflammatory responses. During chronic and acute inflammation, superoxide (controlled by MnSOD) production is in a high rate, which overthrows the SOD defensive system to remove it. Superoxide combines with nitric oxide forming peroxynitrite that is a potent cytotoxic pro-inflammatory mediator. A result of increased levels of peroxynitrite is nitration of MnSOD, which inhibits its biological function leading to enhanced inflammation and pain response.

Aim: To investigate if idebenone, a free radical scavenger on the market for the treatment of different neurological disorders, can be used as a drug for the treatment of inflammatory pain.

Materials and Methods: Nine groups consisting of five rats per group was used. Determination of hyperalgesic response to heat was done accordingly to the Hargreaves method. Paw edema has been detected by Plethismometor analysis. The lumbar tract of the rat spinal cord have been collected and Western blot was performed to determine the expression of MnSOD and an immunoprecipitation assay was done to determine the amount of nitrated MnSOD. Furthermore, Malonildhialdeide (MDA), a by-product of lipid peroxidation, has been measured.

Results: The results show that the cyclodextrine-formulated idebenone significantly reduces edema and thermal hyperalgesia in a dose-dependent manner. Results also show that idebenone inhibits the posttranslational nitration of MnSOD and MDA formation.

Conclusions: Initial investigations show that cyclodextrine-formulated idebenone can be used as a drug for the treatment of inflammatory pain. 

Place, publisher, year, edition, pages
2015. , 29 p.
Keyword [en]
idebenone, pain, inflammatory pain, inflammation.
National Category
Pharmaceutical Sciences
URN: urn:nbn:se:uu:diva-255122OAI: oai:DiVA.org:uu-255122DiVA: diva2:820967
External cooperation
Universita degli studi di Roma Tor Vergata.
Subject / course
Educational program
Bachelor of Science Programme in Pharmacy
Available from: 2015-06-15 Created: 2015-06-13 Last updated: 2015-06-15Bibliographically approved

Open Access in DiVA

No full text

By organisation
Department of Pharmaceutical Biosciences
Pharmaceutical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 271 hits
ReferencesLink to record
Permanent link

Direct link