The Abundant Free Living Amoeba, Acanthamoeba polyphaga, Increases the Survival of Campylobacter jejuni in Milk and Orange Juice
(English)Manuscript (preprint) (Other academic)
Campylobacter jejuni is a common cause of human bacterial diarrhea in most parts of the world. Most C. jejuni infections are acquired from contaminated poultry, milk and water. Due to health care costs and human suffering, it is important to identify all possible sources of infection. Unpasteurized milk has been associated with several outbreaks of C. jejuni infection. Campylobacter has been identified on fresh fruit and other gastrointestinal pathogens such as Salmonella, E. coli O157:H7 and Cryptosporidium have been involved in fruit juice outbreaks. C. jejuni is sensitive to the acidic environment of fruit juice, but co-cultures with A. polyphaga have previously been shown to protect C. jejuni at low pH. In this study we found that co-culture with A. polyphaga prolonged the C. jejuni survival both in milk and juice. The effect of co-culture was most pronounced in juice stored at room temperature. On the other hand, we found that A. polyphaga did not have any effect on C. jejuni survival during pasteurization of milk or orange juice, indicating that this is a good method for eliminating C. jejuni in these products. In conclusion, amoebae associated C. jejuni in milk and juice might cause C. jejuni infections.
juice, unpasteurized milk, Campylobacter, Acanthamoeba
Medical and Health Sciences
Research subject Infectious Diseases
IdentifiersURN: urn:nbn:se:uu:diva-255770OAI: oai:DiVA.org:uu-255770DiVA: diva2:823557
FunderSwedish Research Council Formas, 2220-8779-120, 221-2012-1442