Remediation of diclofenac in a non-sterile bioreactor using the white rot fungus Trametes versicolor
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
From an environmental perspective, it is interesting to assess new methods for efficient removal of drugs from wastewater. The purpose of this project was to assess the possibility of using the white rot fungus Trametes versicolor to degrade diclofenac in a lab scale bioreactor. Two methods for quantitative analysis of diclofenac were developed, using GC-MS and UHPLC-Q-TOF (C18-column). Both methods were partly validated, with regard to sensitivity, linearity, accuracy and precision, which highlighted the superiority of UHPLC-Q-TOF over GC-MS. Two HILIC columns were also assessed, but proved unsuitable for quantitative analysis of diclofenac under the used conditions. The fungal mycelia were immobilized on plastic carriers in a nutrient solution. In initial E-flask experiments, 10 mg/L diclofenac was added to an active culture and a heat-killed control of T. versicolor . Samples were analyzed, and the results from the active culture indicated a 98% removal of diclofenac after 48 hours. The lab scale bioreactor was used in a semi-continuous mode with the influent containing 10 mg/L diclofenac. Samples were collected from the effluent to monitor the concentration over 7 days. The results showed a decline in concentration to a stable level of approximately 2 mg/L. The initial experiments showed that most of the removal (85%) was due to sorption of diclofenac, but a clear difference was seen between the active and heat-killed culture. It was impossible to conclude from the bioreactor experiment if the observed removal was due to sorption or to a combination of sorption and enzymatic remediation.
Place, publisher, year, edition, pages
2015. , 25 p.
UPTEC K, ISSN 1650-8297 ; 15021
Other Chemical Engineering
IdentifiersURN: urn:nbn:se:uu:diva-254554OAI: oai:DiVA.org:uu-254554DiVA: diva2:824944
Master Programme in Chemical Engineering
Pettersson, CurtEngskog, Mikael