Suppression of the neoplastic phenotype by transfection of phospholipase C3 to neuroendocrine tumor cells
1999 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 450, no 3, 210-216 p.Article in journal (Refereed) Published
The expression of phospholipase C beta 3 (PLCB3) is low or absent in several neuroendocrine neoplasias. To investigate the role of PLCB3 in the neuroendocrine tumorigenesis, we transfected a PLCB3 construct to three neuroendocrine tumor cell lines with a low PLCB3 expression. The growth rate and tumorigenicity were assessed in vitro by [3H]thymidine incorporation and cell counting, in vivo, by xenografting to nude mice. In vitro, PLCB3 expressing clones showed a significant growth inhibition. The tumor weight was reduced for one of the two xenografted PLCB3-transfected cell lines and in both, a reduced number of proliferating (Ki-67 positive) cells was observed. This study implies an essential role for PLCB3 in the neuroendocrine tumorigenesis.
Place, publisher, year, edition, pages
1999. Vol. 450, no 3, 210-216 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-54594DOI: 10.1016/S0014-5793(99)00457-3PubMedID: 10359076OAI: oai:DiVA.org:uu-54594DiVA: diva2:82503