IL-5 priming of the PMA-induced oxidative metabolism of human eosinophils from allergic and normal subjects during a pollen season
2001 (English)In: Clinical and Experimental Allergy, ISSN 0954-7894, E-ISSN 1365-2222, Vol. 31, no 4, 555-564 p.Article in journal (Refereed) Published
AIM: To study the effect of IL-5 priming on the PMA-induced oxidative metabolism of blood eosinophils from allergic patients and healthy controls, during pollen exposure.
METHODS: Twenty birch pollen allergic patients with seasonal symptoms of rhinitis or rhinitis plus asthma were studied during the birch pollen season of Sweden. Eosinophils were purified to > 95% by Percoll gradients followed by the MACS system. Oxidative metabolism was measured by a lucigenin enhanced chemiluminescence (CL) assay. Eosinophils were primed with IL-5 and subsequently stimulated with PMA. The signal transduction mechanisms of IL-5 priming were studied using the MEK inhibitor PD 98059, the PkC inhibitors Staurosporine, Ro 318220, Gö 6983 and the PI3kinase inhibitor Wortmannin.
RESULTS: During the season, the eosinophils from the allergic patients showed a reduced t(1/2)rise compared to the non-allergic controls (P = 0.019) after stimulation. IL-5 reduced the total PMA CL response both in control and patients' cells (P = 0.012 and 0.0054 resp.), whereas it primed it in terms of the t(1/2)rise of the curves, in both groups (P = 0.012 and 0.0015 resp.). The PMA-induced CL reactions were inhibited by PD 98059, all PkC-inhibitors and Wortmannin. IL-5 priming counteracted only the MEK inhibition significantly.
CONCLUSIONS: Blood eosinophils from allergic patients are primed in vivo, as compared to eosinophils from non-allergic controls, during a pollen season. Interleukin-5 primes equally the PMA-induced oxidative metabolism of human eosinophils from healthy or allergic subjects. The mechanism of IL-5 priming after PMA stimulation of oxygen radical production is MEK independent.
Place, publisher, year, edition, pages
2001. Vol. 31, no 4, 555-564 p.
eosinophils, priming, IL-5, PMA, asthma, rhinitis, CL, signal transduction, oxidative metabolism
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-54802DOI: 10.1046/j.1365-2222.2001.00995.xPubMedID: 11359422OAI: oai:DiVA.org:uu-54802DiVA: diva2:82711