Adult human osteoblast-like cells do not express insulin-like growthfactor-I
1994 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, Vol. 199, no 1, 78-82 p.Article in journal (Refereed) Published
Previous studies using cultures of fetal rodents calvaria have indicated an important role for insulin-like growth factor-I (IGF-I) in the local control of bone formation. In this study, we have examined the expression of IGF-I in adult human osteoblast-like (hOB) cells. To detect very low levels and to distinguish between the two IGF-I mRNA transcripts Ea and Eb, which are formed by alternate splicing, we have used the reverse transcriptase-polymerase chain reaction (RT-PCR). Expression of both Ea and Eb IGF-I mRNA transcripts were found in human liver and in placenta. However, neither Ea nor Eb IGF-I mRNA could be detected under basal conditions or after stimulation with growth hormone in normal hOB cells and two human osteosarcoma cell lines with osteoblastic properties, SaOS-2 and MG-63. We conclude that adult hOB cells do not synthesize IGF-I. Thus, in contrast to its crucial role as a local regulator of skeletal remodeling in fetal rodent bone, IGF-I does not appear to have this autocrine function in adult human bone.
Place, publisher, year, edition, pages
1994. Vol. 199, no 1, 78-82 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-54899DOI: 10.1006/bbrc.1994.1196PubMedID: 8123049OAI: oai:DiVA.org:uu-54899DiVA: diva2:82808