Reverse transcription-polymerase chain reaction assay demonstrates thatthe 9-cis retinoic acid receptor alpha is expressed in human osteoblasts
1993 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, Vol. 192, no 3, 1367-1372 p.Article in journal (Refereed) Published
The 9-cis retinoic acid receptor (RXR) alpha, a co-regulator of the thyroid hormone and vitamin D receptors, has previously been shown to be expressed predominantly in metabolic organs such as the liver and the kidney. In this study we have used a reverse transcription polymerase chain reaction (RT-PCR) to examine the expression of retinoic acid (RA) nuclear receptors in primary human osteoblasts and in SaOS-2, a human osteosarcoma-derived cell line with osteoblastic characteristics. Our results demonstrate that human osteoblasts express RXR alpha, as well as the all-trans RA receptors (RAR) alpha, beta, and gamma. These data further establish bone as a major target for retinoids and suggest that RA can regulate vitamin D and thyroid hormone actions in osteoblasts.
Place, publisher, year, edition, pages
1993. Vol. 192, no 3, 1367-1372 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-54900DOI: 10.1006/bbrc.1993.1567PubMedID: 7685168OAI: oai:DiVA.org:uu-54900DiVA: diva2:82809