A genetically engineered purpurin/retinol-binding protein hybrid that binds to transthyretin
1992 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, Vol. 184, no 2, 938-944 p.Article in journal (Refereed) Published
A mini-gene encoding rat retinol-binding protein (RBP) and a cDNA encoding chicken purpurin were separately transfected into HeLa cells. In contrast to RBP, expressed purpurin did not bind to transthyretin (TTR). A purpurin/RBP hybrid protein was constructed by substituting the cDNA sequence encoding the N-terminal 29 amino acids of purpurin for the corresponding part of RBP. The expressed hybrid molecule bound to the TTR-Sepharose. These results demonstrate that purpurin does not bind to TTR, that a functional purpurin/RBP hybrid can be constructed, and that the N-terminal coil of RBP is not required for TTR binding.
Place, publisher, year, edition, pages
1992. Vol. 184, no 2, 938-944 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-54904DOI: 10.1016/0006-291X(92)90681-APubMedID: 1575761OAI: oai:DiVA.org:uu-54904DiVA: diva2:82813