Growth hormone and insulin-like growth factor-I do not activate identical genes in normal human osteoblasts
1996 (English)In: Biochemistry and Molecular Biology International, ISSN 1039-9712, Vol. 38, no 2, 425-428 p.Article in journal (Refereed) Published
In this study, we have examined the expression of mRNAs induced by growth hormone (GH) and insulin-like growth factor-I (IGF-I) in normal human osteoblasts using the differential display PCR method. Cells were incubated in the presence of 10(-7)M GH or IGF-I, RNA isolated and reverse-transcribed using an oligonucleotide primer, T11AC, anchored to the polyadenylate tail of a subset of mRNAs. The cDNA was then amplified using T11AC and an arbitrary 10-mer in the presence of [alpha-35S] dCTP. The PCR products were subsequently separated on a 6% DNA sequencing gel. The obtained patterns demonstrate that most, but not all, mRNAs induced by GH or IGF-I are identical. We conclude that GH and IGF-I do not induce expression of identical mRNAs in human osteoblasts. Our results support the dual effector theory of growth hormone action.
Place, publisher, year, edition, pages
1996. Vol. 38, no 2, 425-428 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-55165PubMedID: 8850539OAI: oai:DiVA.org:uu-55165DiVA: diva2:83073