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Cloning of a novel retinoic acid-inducible mRNA from human osteoblast-like cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Internal Medicine. (osteo)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Internal Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Internal Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Internal Medicine. (osteo)
1997 (English)In: Biochemistry and Molecular Biology International, ISSN 1039-9712, Vol. 43, no 5, 1145-1150 p.Article in journal (Refereed) Published
Abstract [en]

Retinoids have long been known to influence skeletal development and bone remodeling. Cells of the osteoblastic lineage play a key role in these processes. In this study we have used the differential display PCR technique to identify retinoic acid (RA)-induced mRNAs in human osteoblast-like cells. We report the cloning and sequencing of one such mRNA, AT-RA 6, which was specifically induced by all-trans RA both in normal human osteoblast-like cells and in MG-63 osteosarcoma cells. Maximal expression was found after 60 min, suggesting that this may be an early response gene. Expression was found in all tissues examined. No homology to known mRNA sequences was detected.

Place, publisher, year, edition, pages
1997. Vol. 43, no 5, 1145-1150 p.
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-55166DOI: 10.1080/15216549700204971PubMedID: 9415824OAI: oai:DiVA.org:uu-55166DiVA: diva2:83074
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2017-12-04Bibliographically approved

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Melhus, HåkanWilén, BirgittaKindmark, AndreasLjunghall, Sverker

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