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Malignant Cell-Derived Extracellular Vesicles Express Different Chromogranin Epitopes Compared to Prostasomes
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemial structure and function.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemical endocrinology.
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2015 (English)In: The Prostate, ISSN 0270-4137, E-ISSN 1097-0045, Vol. 75, no 10, 1063-1073 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND. Prostasomes are nanosized extracellular vesicles exocytosed by prostate epithelial cells. They have been assigned many roles propitious to sperm in favor of fertilization. Prostatic cancer cells can also produce and secrete extracellular vesicles. METHODS. We assessed using ELISA, the surface expression of chromogranin proproteins on prostasomes and malignant extracellular vesicles of four different prostate cancer cell-lines, two hormone sensitive and two hormone refractory. We used a panel of chromogranin A and chromogranin B antibodies against peptides in-between hypothetical cleavage sites along the proproteins. RESULTS. A diverging pattern of chromogranin peptides was apparent when comparing prostasomes and malignant extracellular vesicles indicating a phenotypical change. We also compared western blot patterns (prostasomes and malignant extracellular vesicles) for selected antibodies that displayed high absorbances in the ELISA. Western blot analyses revealed various cleavage patterns of those proproteins that were analyzed in prostasomes and extracellular vesicles. CONCLUSION. Chromogranins are constituents of not only prostasomes but also of malignant prostate cell-derived extracellular vesicles with different amino acid sequences exposed at the membrane surface giving rise to a mosaic pattern. These findings may be of relevance for designing new assays for detection or even possible treatment of prostate cancers.

Place, publisher, year, edition, pages
2015. Vol. 75, no 10, 1063-1073 p.
Keyword [en]
prostasomes, extracellular vesicles, PC3, LNCaP, DU145, chromogranins, synaptophysin, prostate cancer
National Category
Endocrinology and Diabetes Urology and Nephrology
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URN: urn:nbn:se:uu:diva-256204DOI: 10.1002/pros.22990ISI: 000354203600006PubMedID: 25783430OAI: oai:DiVA.org:uu-256204DiVA: diva2:840050
Available from: 2015-07-06 Created: 2015-06-22 Last updated: 2017-12-04Bibliographically approved

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Dubois, LouiseStridsberg, MatsRonquist, Göran

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Biochemial structure and functionBiochemical endocrinology
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