uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Hypoxanthine, uric acid and allantoin as indicators of in vivo free radical reactions. Description of a HPLC method and human brain microdialysis data.
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience.
Department of Surgical Sciences. (Orthopaedics)
Department of Medical Sciences.
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience.
Show others and affiliations
2000 (English)In: Acta Neurochir (Wien), Vol. 142Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2000. Vol. 142
URN: urn:nbn:se:uu:diva-56099OAI: oai:DiVA.org:uu-56099DiVA: diva2:84007
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14
In thesis
1. Influence of Oxidative Stress on Muscle and Bone
Open this publication in new window or tab >>Influence of Oxidative Stress on Muscle and Bone
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Reactive oxygen species (ROS) induce oxidative stress and although are primarily recognized for playing a deleterious biological role, they can be beneficial to cell systems. ROS are extremely short-lived and normally tightly regulated by antioxidant defence systems. Cells react to oxidative stress in different ways, which primarily depends on cell type, stress severity, or both. There is a general limitation in extrapolating to humans conclusions drawn from in vitro and animal studies because of important species-specific differences. Therefore, the general aim of this thesis was to study the influence of oxidative stress on human muscle and bone in vivo.

In paper I we presented a one-step HPLC method optimized for the simultaneous determination of purine degradation products in small microdialysis samples. The clinical utility of the method was successfully tested in a patient with traumatic brain injury. In paper II we evaluated microdialysis as an in vivo method to characterize the relative kinetics of ROS-related metabolites in human skeletal muscle exposed to ischaemia-reperfusion. Results indicated that microdialysis was feasible and safe to use in monitoring metabolic events during tourniquet-assisted surgery. In paper III we investigated the association between an oxidative stress marker (urinary 8-iso-PGF) and bone mineral density (BMD) and whether α-tocopherol modified the association. The main finding was the negative association between 8-iso-PGF and BMD and that the association was further dependent on serum α-tocopherol level. In paper IV we performed a randomized controlled trial to evaluate the influence of Q10 supplementation on exercise performance and metabolites of muscular damage. We did not observe any effects on exercise capacity after 8 weeks of Q10 administration. Nor did we find a significant effect on serum markers related to oxidative stress.

In conclusion we have studied the influence of oxidative stress on muscle and bone in vivo in humans. The oxidative stress was triggered by four different causes (trauma, ischemia-reperfusion, ageing, and exercise exhaustion).

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 72 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 501
bone mineral density, coenzyme Q10, exercise, ischaemia, isprostanes, muscle, oxidative stress, oxygen radicals, reperfusion, tocopherol
National Category
Research subject
urn:nbn:se:uu:diva-110093 (URN)978-91-554-7661-8 (ISBN)
Public defence
2009-12-17, Rosénsalen, Barnkliniken, Akademiska sjukhuset, Uppsala, 13:00 (Swedish)
Available from: 2009-11-25 Created: 2009-11-03 Last updated: 2009-11-25Bibliographically approved

Open Access in DiVA

No full text

By organisation
Department of NeuroscienceDepartment of Surgical SciencesDepartment of Medical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 168 hits
ReferencesLink to record
Permanent link

Direct link