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Sfp-type PPTase inactivation promotes bacterial biofilm formation and ability to enhance wheat drought tolerance
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2015 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 6, 387Article in journal (Refereed) Published
Abstract [en]

Paenibacillus polymyxa is a common soil bacterium with broad range of practical applications. An important group of secondary metabolites in P polymyxa are non-ribosomal peptide and polyketide derived metabolites (NRPs/PKs). Modular non-ribosomal peptide synthetases catalyze main steps in the biosynthesis of the complex secondary metabolites. Here we report on the inactivation of an A26 Sfp-type 4'-phosphopantetheinyl transferase (Sfp-type PPTase). The inactivation of the gene resulted in loss of NRPs/PKs production. In contrast to the former Bacillus spp. model the mutant strain compared to wild type showed greatly enhanced biofilm formation ability. A26 Delta sfp biofilm promotion is directly mediated by NRPs/PKs, as exogenous addition of the wild type metabolite extracts restores its biofilm formation level. Wheat inoculation with bacteria that had lost their Sfp-type PPTase gene resulted in two times higher plant survival and about three times increased biomass under severe drought stress compared to wild type. Challenges with P. polymyxa genetic manipulation are discussed.

Place, publisher, year, edition, pages
2015. Vol. 6, 387
Keyword [en]
Sfp-type PPTase, Paenibacillus polymyxa, Evolution Canyon, rhizobacterial biofilm, plant drought tolerance, natural isolate genetic manipulation
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-258051DOI: 10.3389/fmicb.2015.00387ISI: 000356266100001PubMedID: 26052312OAI: oai:DiVA.org:uu-258051DiVA: diva2:841408
Funder
EU, FP7, Seventh Framework Programme, FP7:247669Swedish Research Council Formas, 2009-243Swedish Research Council, 2012-6217Carl Tryggers foundation , CTS09:385
Available from: 2015-07-13 Created: 2015-07-10 Last updated: 2017-12-04Bibliographically approved

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Ek, BoBergquist, Jonas

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