uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Show others and affiliations
2015 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, 11196Article in journal (Refereed) Published
Abstract [en]

Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS.

Place, publisher, year, edition, pages
2015. Vol. 5, 11196
National Category
Medical Biotechnology
Identifiers
URN: urn:nbn:se:uu:diva-258341DOI: 10.1038/srep11196ISI: 000356508700001OAI: oai:DiVA.org:uu-258341DiVA: diva2:841521
Available from: 2015-07-13 Created: 2015-07-13 Last updated: 2017-12-04Bibliographically approved

Open Access in DiVA

fulltext(545 kB)246 downloads
File information
File name FULLTEXT01.pdfFile size 545 kBChecksum SHA-512
ea7ab9df7caf1fdef04311e7c780d4964053c28a314a29cb83176729d43498f4e83c350c4568aebce49b4c66ea1d5da1078ddad630bcaa5fa7c5650b2ba1053f
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Gu, Gucci JijuanLandegren, UlfKamali-Moghaddam, Masood

Search in DiVA

By author/editor
Gu, Gucci JijuanLandegren, UlfKamali-Moghaddam, Masood
By organisation
Science for Life Laboratory, SciLifeLabMolecular toolsDepartment of Immunology, Genetics and Pathology
In the same journal
Scientific Reports
Medical Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 246 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 2541 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf