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Differential transcriptional regulation of orthologous dps genes from two closely related heterocyst-forming cyanobacteria
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science, Microbial Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science, Microbial Chemistry. (Karin Stensjö)
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics.
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2015 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 362, no 6, fnv017Article in journal (Refereed) Published
Abstract [en]

In cyanobacteria, DNA-binding proteins from starved cells (Dps) play an important role in the cellular response to oxidative and nutritional stresses. In this study, we have characterized the cell-type specificity and the promoter regions of two orthologous dps genes, Npun_R5799 in Nostoc punctiforme and alr3808 in Anabaena sp. PCC 7120. A transcriptional start site (TSS), identical in location to the previously identified proximal TSS of alr3808, was identified for Npun_R5799 under both combined nitrogen supplemented and N-2-fixing growth conditions. However, only alr3808 was also transcribed from a second distal TSS. Sequence homologies suggest that the promoter region containing the distal TSS is not conserved upstream of orthologous genes among heterocyst-forming cyanobacteria. The analysis of promoter GFP-reporter strains showed a different role in governing cell-type specificity between the proximal and distal promoter of alr3808. We here confirmed the heterocyst specificity of the distal promoter of alr3808 and described a very early induction of its expression during proheterocyst differentiation. In contrast, the complete promoters of both genes were active in all cells. Even though Npun_R5799 and alr3808 are orthologs, the regulation of their respective expression differs, indicating distinctions in the function of these cyanobacterial Dps proteins depending on the strain and cell type.

Place, publisher, year, edition, pages
2015. Vol. 362, no 6, fnv017
Keyword [en]
Cyanobacteria, Nostoc, Anabaena, heterocyst differentiation, ferritin-like protein, ROS scavenging, DIF motif
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-258897DOI: 10.1093/femsle/fnv017ISI: 000356885900005OAI: oai:DiVA.org:uu-258897DiVA: diva2:842600
Funder
Knut and Alice Wallenberg FoundationCarl Tryggers foundation
Available from: 2015-07-21 Created: 2015-07-21 Last updated: 2017-12-04

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Li, XinSandh, GustafMuro-Pastor, Alicia M.Stensjo, Karin

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