uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Biochanin A Gastroprotective Effects in Ethanol-Induced Gastric Mucosal Ulceration in Rats
Show others and affiliations
2015 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 10, no 3, e0121529Article in journal (Refereed) Published
Abstract [en]

Background Biochanin A notable bioactive compound which is found in so many traditional medicinal plant. In vivo study was conducted to assess the protective effect of biochanin A on the gastric wall of Spraguedawley rats' stomachs. Methodology The experimental set included different animal groups. Specifically, four groups with gastric mucosal lesions were receiving either a) Ulcer control group treated with absolute ethanol (5 ml/kg), b) 20 mg/kg of omeprazole as reference group, c) 25 of biochanin A, d) 50 mg/kg of biochanin A. Histopathological sectioning followed by immunohistochemistry staining were undertaken to evaluate the influence of the different treatments on gastric wall mucosal layer. The gastric secretions were collected in the form of homogenate and exposed to superoxide dismutase (SOD) and nitric oxide enzyme (NO) and the level of malondialdehyde (MDA) and protein content were measured. Ulceration and patchy haemorrhage were clearly observed by light microscopy. The morphology of the gastric wall as confirmed by immunohistochemistry and fluorescent microscopic observations, exhibited sever deformity with notable thickness, oedematous and complete loss of the mucosal coverage however the biochanin-pretreated animals, similar to the omeprazole-pretreated animals, showed less damage compared to the ulcer control group. Moreover, up-regulation of Hsp70 protein and down-regulation of Bax protein were detected in the biochanin A pre-treated groups and the gastric glandular mucosa was positively stained with Periodic Acid Schiff (PAS) staining and the Leucocytes infiltration was commonly seen. Biochanin A displayed a great increase in SOD and NO levels and decreased the release of MDA. Conclusions This gastroprotective effect of biochanin A could be attributed to the enhancement of cellular metabolic cycles perceived as an increase in the SOD, NO activity, and decrease in the level of MDA, and also decrease in level of Bax expression and increase the Hsp70 expression level.

Place, publisher, year, edition, pages
2015. Vol. 10, no 3, e0121529
National Category
Medicinal Chemistry
URN: urn:nbn:se:uu:diva-258895DOI: 10.1371/journal.pone.0121529ISI: 000356353700111PubMedID: 25811625OAI: oai:DiVA.org:uu-258895DiVA: diva2:842624
Available from: 2015-07-21 Created: 2015-07-21 Last updated: 2015-07-21Bibliographically approved

Open Access in DiVA

fulltext(4828 kB)49 downloads
File information
File name FULLTEXT01.pdfFile size 4828 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed
By organisation
Division of Pharmacognosy
In the same journal
Medicinal Chemistry

Search outside of DiVA

GoogleGoogle Scholar
Total: 49 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 227 hits
ReferencesLink to record
Permanent link

Direct link