uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Function of Isolated Pancreatic Islets From Patients at Onset of Type 1 Diabetes: Insulin Secretion Can Be Restored After Some Days in a Nondiabetogenic Environment In Vitro: Results From the DiViD Study
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Show others and affiliations
2015 (English)In: Diabetes, ISSN 0012-1797, E-ISSN 1939-327X, Vol. 64, no 7, 2506-2512 p.Article in journal (Refereed) Published
Abstract [en]

The understanding of the etiology of type 1 diabetes (T1D) remains limited. One objective of the Diabetes Virus Detection (DiViD) study was to collect pancreatic tissue from living subjects shortly after the diagnosis of T1D. Here we report the insulin secretion ability by in vitro glucose perifusion and explore the expression of insulin pathway genes in isolated islets of Langerhans from these patients. Whole-genome RNA sequencing was performed on islets from six DiViD study patients and two organ donors who died at the onset of T1D, and the findings were compared with those from three nondiabetic organ donors. All human transcripts involved in the insulin pathway were present in the islets at the onset of T1D. Glucose-induced insulin secretion was present in some patients at the onset of T1D, and a perfectly normalized biphasic insulin release was obtained after some days in a nondiabetogenic environment in vitro. This indicates that the potential for endogenous insulin production is good, which could be taken advantage of if the disease process was reversed at diagnosis.

Place, publisher, year, edition, pages
2015. Vol. 64, no 7, 2506-2512 p.
National Category
Endocrinology and Diabetes
URN: urn:nbn:se:uu:diva-259101DOI: 10.2337/db14-1911ISI: 000356934000033PubMedID: 25677915OAI: oai:DiVA.org:uu-259101DiVA: diva2:843451
Swedish Diabetes Association

De tre första författarna delar förstaförfattarskapet.

De sista två författarna delar sistaförfattarskapet.

Available from: 2015-07-28 Created: 2015-07-27 Last updated: 2016-01-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Skog, OskarSundström, GörelGrabherr, ManfredKorsgren, Olle
By organisation
Clinical ImmunologyDepartment of Medical Biochemistry and Microbiology
In the same journal
Endocrinology and Diabetes

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 373 hits
ReferencesLink to record
Permanent link

Direct link