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A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemial structure and function.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemial structure and function.
2015 (English)In: Journal of Inflammation, ISSN 1476-9255, E-ISSN 1476-9255, Vol. 12, 45Article in journal (Refereed) Published
Abstract [en]

Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was developed based on calprotectin values observed in samples from the intensive care unit. The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens. The performance was tested and it was observed that the assay was linear in the range 0.3-24.7 mg/L, the limit of quantitation was observed to be lower than 0.3 mg/L, no antigen excess was observed up to 54 mg/L, all CVs were lower than 1.8 % in the precision study, the calibration curve stability was longer than 6 weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin. The serum calprotectin immunoassay presented in this paper performs well within the criteria carefully set from the limited clinical experience obtained in both serum and plasma. In addition it is commutable with Bühlmann MRP8/14 ELISA.

Place, publisher, year, edition, pages
2015. Vol. 12, 45
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:uu:diva-259346DOI: 10.1186/s12950-015-0090-3ISI: 000358486900002PubMedID: 26213499OAI: oai:DiVA.org:uu-259346DiVA: diva2:843856
Note

Economical support was given from the Norwegian Research Council.

Available from: 2015-07-31 Created: 2015-07-31 Last updated: 2017-12-04Bibliographically approved

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Nilsen, TomLarsson, Anders

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