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Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism.
Department of Plant and Microbial Biology, University of California, Berkeley.ORCID iD: 0000-0002-6413-1443
Department of Plant and Microbial Biology, University of California, Berkeley.
Department of Plant and Microbial Biology, University of California, Berkeley.
2010 (English)In: Metabolic engineering, ISSN 1096-7176, E-ISSN 1096-7184, Vol. 12, no 1, p. 70-9Article in journal (Refereed) Published
Abstract [en]

The concept of "photosynthetic biofuels" envisions application of a single organism, acting both as photo-catalyst and producer of ready-made fuel. This concept was applied upon genetic engineering of the cyanobacterium Synechocystis, conferring the ability to generate volatile isoprene hydrocarbons from CO(2) and H(2)O. Heterologous expression of the Pueraria montana (kudzu) isoprene synthase (IspS) gene in Synechocystis enabled photosynthetic isoprene generation in these cyanobacteria. Codon-use optimization of the kudzu IspS gene improved expression of the isoprene synthase in Synechocystis. Use of the photosynthesis psbA2 promoter, to drive the expression of the IspS gene, resulted in a light-intensity-dependent isoprene synthase expression. Results showed that oxygenic photosynthesis can be re-directed to generate useful small volatile hydrocarbons, while consuming CO(2), without a prior requirement for the harvesting, dewatering and processing of the respective biomass.

Place, publisher, year, edition, pages
2010. Vol. 12, no 1, p. 70-9
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-259991DOI: 10.1016/j.ymben.2009.10.001PubMedID: 19833224OAI: oai:DiVA.org:uu-259991DiVA, id: diva2:845950
Available from: 2015-08-13 Created: 2015-08-13 Last updated: 2017-12-04

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