uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
In vitro blood-brain barrier models: An overview of established models and new microfluidic approaches
Lund University.
Lund University.
Copenhagen University.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.ORCID iD: 0000-0002-1264-1337
2015 (English)In: Journal of Pharmaceutical Sciences, ISSN 0022-3549, E-ISSN 1520-6017, Vol. 104, no 9, 2727-2746 p.Article in journal (Refereed) Published
Abstract [en]

The societal need for new central nervous system (CNS) medicines is substantial, because of the global increase in life expectancy and the accompanying increase in age-related CNS diseases. Low blood-brain barrier (BBB) permeability has been one of the major causes of failure for new CNS drug candidates. There has therefore been a great interest in cell models, which mimic BBB permeation properties. In this review, we present an overview of the performance of monocultured, cocultured, and triple-cultured primary cells and immortalized cell lines, including key parameters such as transendothelial electrical resistance values, permeabilities of paracellular flux markers, and expression of BBB-specific marker proteins. Microfluidic systems are gaining ground as a new automated technical platform for cell culture and systematic analysis. The performance of these systems was compared with current state-of-the-art models and it was noted that, although they show great promise, these systems have not yet reached beyond the proof-of-concept stage. In general, it was found that there were large variations in experimental protocols, BBB phenotype markers, and paracellular flux markers used. It is the author's opinion that the field may benefit greatly from developing standardized methodologies and initiating collaborative efforts on optimizing culture protocols.

Place, publisher, year, edition, pages
2015. Vol. 104, no 9, 2727-2746 p.
National Category
Pharmacology and Toxicology Medicinal Chemistry Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
URN: urn:nbn:se:uu:diva-260031DOI: 10.1002/jps.24329ISI: 000359857200007PubMedID: 25630899OAI: oai:DiVA.org:uu-260031DiVA: diva2:846061
Swedish Research Council Formas
Available from: 2015-08-14 Created: 2015-08-14 Last updated: 2015-10-23Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Tenje, Maria
By organisation
Microsystems Technology
In the same journal
Journal of Pharmaceutical Sciences
Pharmacology and ToxicologyMedicinal ChemistryEngineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 448 hits
ReferencesLink to record
Permanent link

Direct link