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Formation of Silver Nanostructures by Rolling Circle Amplification Using Boranephosphonate-Modified Nucleotides
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools.
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2015 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 87, no 13, 6660-6666 p.Article in journal (Refereed) Published
Abstract [en]

We investigate the efficiency of incorporation of boranephosphonate-modified nucleotides by phi29 DNA poly, merase and present a simple method for forming large defined silver nanostructures by rolling circle amplification (RCA) using boranephosphonate internudeotide linkages. RCA is a linear DNA amplification technique that can use specifically circularized DNA probes for detection of target nucleic acids and proteins. The resulting product is a collapsed single-stranded DNA molecule with tandem repeats of the DNA probe. By substituting each of the natural nucleotides with the corresponding 5'-(alpha-P-borano)-deoxynudeosidetriphosphate, only a small reduction in amplification rate is observed. Also, by substituting all four natural nucleotides, it is possible to enzymatically synthesize a micrometensized, single-stranded DNA molecule with only boranephosphonate internucleotide linkages. Well-defined silver particles are then readily formed along the rolling circle product.

Place, publisher, year, edition, pages
2015. Vol. 87, no 13, 6660-6666 p.
National Category
Analytical Chemistry
URN: urn:nbn:se:uu:diva-260302DOI: 10.1021/acs.analchem.5b00783ISI: 000357839700037PubMedID: 26059318OAI: oai:DiVA.org:uu-260302DiVA: diva2:847612
VINNOVASwedish Research Council
Available from: 2015-08-20 Created: 2015-08-18 Last updated: 2015-08-20Bibliographically approved

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