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Isolation of buffalo poxvirus from clinical case and variations in the genetics of the B5R gene over fifty passages
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2015 (English)In: Virus genes, ISSN 0920-8569, E-ISSN 1572-994X, Vol. 51, no 1, 45-50 p.Article in journal (Refereed) Published
Abstract [en]

Outbreaks of buffalopox affect udder and teats, which may ultimately lead to mastitis in dairy buffalo and can significantly compromise the production. In this study, we report isolation of buffalo poxvirus and sequence analysis of the B5R gene collected from the buffalo clinically suspected to be poxvirus infected. The virus was isolated on BHK-21 cell line and was passaged for 50 times, B5R gene was amplified and sequenced using gene-specific primers, and analyzed at both nucleotide and amino acid levels. Phylogenetically, the isolate can be classified close to the previously reported Pakistani and Indian isolates with certain level of differential clustering patterns. Three significant putative mutations (I2K, N64D, and K111E) were observed in the B5R protein. The K111E was common with previous human isolate from Karachi, Pakistan in 2005. These mutations differed from pox-viruses reported from the neighboring countries. Some deletion mutations were observed which were recovered in upcoming passages. The K111E mutation suggests potential to cause zoonotic infection in human all over the country.

Place, publisher, year, edition, pages
2015. Vol. 51, no 1, 45-50 p.
Keyword [en]
Buffalo poxvirus, B5Rgene, Zoonosis, Host range, Phylogeny
National Category
Infectious Medicine Medical Genetics
URN: urn:nbn:se:uu:diva-260827DOI: 10.1007/s11262-015-1209-6ISI: 000358548500006PubMedID: 26070368OAI: oai:DiVA.org:uu-260827DiVA: diva2:848976
Available from: 2015-08-26 Created: 2015-08-25 Last updated: 2015-08-26Bibliographically approved

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