Protein Desalination Chip for Mass Spectrometry Sample Preparation
2015 (English)Conference paper, Abstract (Refereed)
This work focuses on desalination of a protein sample in a lab-on-chip device using the ion concentration polarization (ICP) technique. It was demonstrated with a salt containing buffer with four proteins and two peptides of concentrations typical to cerebrospinal fluid (CSF). Not only was the output desalinated but its protein concentration with large molecular weight (MW) was as much as 3 times higher for the largest protein compared to the original. We conclude that ICP based microfluidic chips have great potential for desalination and protein concentration in microdialysis sampling coupled to mass spectroscopy (MS).
Place, publisher, year, edition, pages
Ion concentration polarization, Desalination, Protein, Mass spectroscopy
Research subject Engineering Science with specialization in Microsystems Technology; Engineering Science with specialization in Materials Science
IdentifiersURN: urn:nbn:se:uu:diva-261067OAI: oai:DiVA.org:uu-261067DiVA: diva2:849551
The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015. October 25-29, 2015. Gyeongju, Korea.