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Response to Comment on “Global diversity and geography of soil fungi”
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Systematic Biology.
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2015 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 349, no 6251, 936- p.Article in journal (Refereed) Published
Abstract [en]

Schadt and Rosling (Technical Comment, 26 June 2015, p. 1438) argue that primer-template mismatches neglected the fungal class Archaeorhizomycetes in a global soil survey. Amplicon-based metabarcoding of nine barcode-primer pair combinations and polymerase chain reaction (PCR)-free shotgun metagenomics revealed that barcode and primer choice and PCR bias drive the diversity and composition of microorganisms in general, but the Archaeorhizomycetes were little affected in the global study. We urge that careful choice of DNA markers and primers is essential for ecological studies using high-throughput sequencing for identification.

Place, publisher, year, edition, pages
2015. Vol. 349, no 6251, 936- p.
National Category
Agricultural Sciences
URN: urn:nbn:se:uu:diva-261107DOI: 10.1126/science.aaa5594ISI: 000360646800036OAI: oai:DiVA.org:uu-261107DiVA: diva2:849679
Available from: 2015-08-30 Created: 2015-08-30 Last updated: 2015-10-13Bibliographically approved

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Publisher's full texthttp://www.sciencemag.org/content/349/6251/936.4.full

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