uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Response to Comment on “Global diversity and geography of soil fungi”
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Systematic Biology.
Show others and affiliations
2015 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 349, no 6251, 936- p.Article in journal (Refereed) Published
Abstract [en]

Schadt and Rosling (Technical Comment, 26 June 2015, p. 1438) argue that primer-template mismatches neglected the fungal class Archaeorhizomycetes in a global soil survey. Amplicon-based metabarcoding of nine barcode-primer pair combinations and polymerase chain reaction (PCR)-free shotgun metagenomics revealed that barcode and primer choice and PCR bias drive the diversity and composition of microorganisms in general, but the Archaeorhizomycetes were little affected in the global study. We urge that careful choice of DNA markers and primers is essential for ecological studies using high-throughput sequencing for identification.

Place, publisher, year, edition, pages
2015. Vol. 349, no 6251, 936- p.
National Category
Agricultural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-261107DOI: 10.1126/science.aaa5594ISI: 000360646800036OAI: oai:DiVA.org:uu-261107DiVA: diva2:849679
Available from: 2015-08-30 Created: 2015-08-30 Last updated: 2017-12-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full texthttp://www.sciencemag.org/content/349/6251/936.4.full

Authority records BETA

Bahram, Mohammad

Search in DiVA

By author/editor
Bahram, Mohammad
By organisation
Systematic Biology
In the same journal
Science
Agricultural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 416 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf