Cellular sexual dimorphism of X and Y homolog gene expression in human central nervous system during early male development
(English)Manuscript (preprint) (Other academic)
Renewed attention has been directed to the functions of the Y chromosome in the central nervous system during early human male development, due to the recent proposed involvement in neurodevelopmental diseases. PCDH11Y and NLGN4Y are of special interest, because they belong to gene families involved in cell fate determination and formation of dendrites and axons. We used RNA sequencing, immunocytochemistry and a padlock probing and rolling circle amplification strategy, to distinguish the expression of X and Y homologs in situ in human brain for the first time. To minimize influence of androgens on the sex differences in the brain, we focused our investigation to human embryos at 8-11 weeks post gestation. The most striking result was that the Y encoded genes are expressed in specific and heterogeneous cellular neural subpopulations that rarely express the X homologs. Our findings suggest that a male-specific cellular network may exist in the embryonic central nervous system.
y chromosome, PCDH11Y, PCDH11X, NLGN4Y, NLGN4Y, spinal cord, medulla oblongata, fetal, embryo, early development, 8 week pcw, male, female, sex differences, expression, cellular, cellular networks, male specific, male specific cellular networks, neun, actb, sox10, olig2, white matter, eppendymal layer, olivary nucleus, padlock, padlock probing, in situ hybridization, rolling circle amplification
Neurosciences Developmental Biology
Research subject Biology with specialization in Molecular Biology; Neurology; Biology with specialization in Evolutionary Organismal Biology
IdentifiersURN: urn:nbn:se:uu:diva-261683OAI: oai:DiVA.org:uu-261683DiVA: diva2:850940
FunderSwedish Research Council, K2012-61X-22089-01-3