MS for investigation of time-dependent protein adsorption on surfaces in complex biological samples
2015 (English)In: Future Science OA, ISSN 2056-5623, 32Article in journal (Refereed) Published
Aim: This study aims at developing a nondestructive way for investigating proteinadsorption on surfaces such as biomaterials using mass spectrometry. Methods: Ventricular cerebrospinal fluid in contact with poly carbonate membranes were usedas adsorption templates and on-surface enzymatic digestion was applied to desorbproteins and cleave them into peptides. Mass spectrometric analysis provided bothprotein identification and determination of protein specific adsorption behavior. Results: In general, the adsorption increased with incubation time but also proteinspecifictime-resolved adsorption patterns from the complex protein solutionwere discovered. Conclusion: The method developed is a promising tool for thecharacterization of biofouling, which sometimes causes rejection and encapsulationof implants and can be used as complement to other surface analytical techniques.
One problem associated with artificial materials in the body is that proteins in thebody interact with the surface, which sometimes causes rejection of the implant.In this study, a method for investigating the time-dependent protein adsorptionon a surface originating from a complex biological protein solution was developed.Compared with other surface analyses, this method can identify what proteins thatadsorbs on the surface. In addition, determination of protein-specific adsorptionbehavior in relation to incubation was possible
Place, publisher, year, edition, pages
Chemical Sciences Engineering and Technology
IdentifiersURN: urn:nbn:se:uu:diva-262022DOI: 10.4155/fso.15.32OAI: oai:DiVA.org:uu-262022DiVA: diva2:851814
FunderÅke Wiberg Foundation, M14–0127Magnus Bergvall Foundation, 2014-00367