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Somatostatin receptor 1-5; expression profiles during rat development
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
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2015 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 120, no 3, p. 157-168Article in journal (Refereed) Published
Description
Abstract [en]

Background. Somatostatin acts through five receptor subtypes (SSTRs 1-5). We aimed to investigate SSTRs mRNA expression and protein distribution in whole rat embryos, with special emphasis on the pancreas. Material and methods. Rat embryos were collected on embryonal days 10, 11, 12, 14, 15, 17, 19, 21, and at birth. Presence of SSTRs was investigated with RT-PCR techniques and immunohistochemistry. Results. There was no SSTR5 mRNA expression in the whole rat embryos. All SSTR1-5 proteins were observed at embryonal day 10, but the localization varied between the different subtypes. From day 11 to birth SSTRs protein presence increased with time in major structures such as skin and cartilage. It remained similar over time in the heart and liver. In the fetal pancreas mRNA expression of SSTR2 and 4 was detected at day 14, and there was an increase up to birth. Only SSTR1 protein co-localized to a higher extent with the islet hormones studied. SSTR2 was present in all islet endocrine cells except for beta-cells. In contrast, the immunostaining for SSTR3-4 was co-localized with insulin and PP, and, finally, SSTR5 with glucagon and pancreatic polypeptide. In mRNA isolated from whole rat embryos SSTR1-2 and SSTR4 expression showed a peak at day 14, while SSTR3 mRNA was not present until day 15. Conclusion. The present data suggest a role for SSTRs during the development of the rat embryo. Subsequent functional studies may elucidate regulatory roles of specific SSTRs for the growth and differentiation of the pancreas as well as other organs.

Place, publisher, year, edition, pages
2015. Vol. 120, no 3, p. 157-168
Keyword [en]
Development, mRNA expression, pancreas, rat embryo, somatostatin receptors
National Category
Endocrinology and Diabetes Cancer and Oncology
Identifiers
URN: urn:nbn:se:uu:diva-261988DOI: 10.3109/03009734.2015.1035413ISI: 000359819800003PubMedID: 25926390OAI: oai:DiVA.org:uu-261988DiVA, id: diva2:852026
Funder
Swedish Research Council, 72X-8273Swedish Diabetes AssociationSwedish Cancer Society
Available from: 2015-09-07 Created: 2015-09-07 Last updated: 2017-12-04Bibliographically approved

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Janson, Eva TiensuuSandler, StellanStridsberg, Mats

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Carlsson, CarinaJanson, Eva TiensuuSandler, StellanStridsberg, Mats
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Department of Medical Cell BiologyDepartment of Medical SciencesBiochemical endocrinology
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