uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Identification of MYCN and SOX9 target genes and a study of drug treatment effects in medulloblastoma
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre. (Fredrik Swartlings forskargrupp, Rudbecklaboratoriet)
2015 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Medulloblastoma (MB) is the most common malignant brain tumor affecting children. The transcription factors MYCN and SOX9 are associated with initiation, maintenance and recurrence of MB and are also connected to more aggressive tumors. In this study, a ChIP was performed to isolate DNA from genes that are transcriptionally regulated by these proteins. Identification of these target genes will reveal new potential drug targets and help us better understand the functions of MYCN and SOX9. The ChIP was not fully optimized during this project and the target genes were not sent for sequencing and identified. To study the connection between SOX9 and recurrence, cells with different levels of SOX9 were treated with drugs, after which cell viability was measured. No significant difference in resistance could be measured. Change in expression level of MYCN, SOX9 and other relevant genes after drug treatment was also studied. The results show an increase in SOX9 and HES1, suggesting that these genes are involved in tumor recurrence.

Place, publisher, year, edition, pages
2015. , 29 p.
UPTEC X, 15 018
Keyword [en]
Medulloblastoma, MYCN, MYC, SOX9, HES1, ChIP, recurrence
National Category
Cancer and Oncology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
URN: urn:nbn:se:uu:diva-262085OAI: oai:DiVA.org:uu-262085DiVA: diva2:852250
Educational program
Molecular Biotechnology Engineering Programme
2015-06-05, 13:38 (English)
Available from: 2015-09-08 Created: 2015-09-08 Last updated: 2015-09-08Bibliographically approved

Open Access in DiVA

fulltext(739 kB)98 downloads
File information
File name FULLTEXT01.pdfFile size 739 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
Biology Education Centre
Cancer and OncologyMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar
Total: 98 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 192 hits
ReferencesLink to record
Permanent link

Direct link