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Roles of miR-196a on gene regulation of neuroendocrine tumor cells
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2015 (English)In: Molecular and Cellular Endocrinology, ISSN 0303-7207, E-ISSN 1872-8057, Vol. 412, no C, 131-139 p.Article in journal (Refereed) Published
Abstract [en]

This study aims at investigating miR-196a roles using in vitro models. miR-196a was detected in small intestinal neuroendocrine tumors (SI-NETS) and lung NETs. miR-196a target prediction analysis suggested HOXA9, HOXB7, LRP4 and RSPO2 genes for further investigation. The level of these four genes is detectable in SI-NET tissue specimens at different disease stages and serum samples of untreated and somatostatin analogs treated patients with liver metastases. A miR-196a inhibitor was used to silence its effects in NET cells. We show that the four target genes were significantly upregulated at transcriptional level in silenced NET cells. HOXA9, HOXB7, LRP4 and RSPO2 encoded proteins are also upregulated at translational level in miR-196a silenced NET cells. miR-196a downstream genes BMP4, ETS1, CTNNB1, FZD5, LEP5 and LRP6 were significantly upregulated at transcriptional level in miR-196a silenced CNDT2.5 and NCI-H727 cells. In addition, miR-196a clearly does not play a role in NET cell growth control.

Place, publisher, year, edition, pages
2015. Vol. 412, no C, 131-139 p.
Keyword [en]
miR-196a target genes, Neuroendocrine tumor cells, MicroRNA inhibitors, Transfection effects
National Category
Endocrinology and Diabetes Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-262406DOI: 10.1016/j.mce.2015.06.003ISI: 000359958900015PubMedID: 26052033OAI: oai:DiVA.org:uu-262406DiVA: diva2:855384
Available from: 2015-09-21 Created: 2015-09-15 Last updated: 2017-12-04Bibliographically approved

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Öberg, KjellGiandomenico, Valeria

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Endocrine oncologyScience for Life Laboratory, SciLifeLab
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Molecular and Cellular Endocrinology
Endocrinology and DiabetesMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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