Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces
2001 (English)In: Laboratory Animals. Journal of the Laboratory Animal Science Association, ISSN 0023-6772, Vol. 35, no 4, 301-306 p.Article in journal (Refereed) Published
Non-invasive measures of immunological markers are an attractive means ofstress assessment in laboratory animals. Salivary IgA has been used successfully as astress marker in the human, and several reports indicate the potential of secretory IgA as anon-invasive measure of stress in animals. The present paper describes the developmentof an ELISA using commercially available components for the quantification of rat IgA andvalidation of this assay for the quantification of rat secretory IgA in saliva and faeces. The concentration of IgA in rat saliva varied significantly between duplicate samples obtained from individual rats, and the viscosity and small total volume of rat saliva gave unsatisfactory results for IgA. Faecal IgA was present in high concentrations, and duplicate samples varied by only 2-3 %. However, faecal IgA seemed less stable than IgA in other biological compartments, and this finding must be taken into consideration when using quantitative measurements of IgA as a marker of mucous humoral immune status.
Place, publisher, year, edition, pages
2001. Vol. 35, no 4, 301-306 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-57783DOI: 10.1258/0023677011911822ISI: 000171394100001OAI: oai:DiVA.org:uu-57783DiVA: diva2:85692