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Exploring microbial dark matter to resolve the deep archaeal ancestry of eukaryotes
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution. Uppsala Univ, Dept Cell & Mol Biol, Sci Life Lab, Uppsala, Sweden..
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2015 (English)In: Philosophical Transactions of the Royal Society of London. Biological Sciences, ISSN 0962-8436, E-ISSN 1471-2970, Vol. 370, no 1678, 20140328Article in journal (Refereed) Published
Abstract [en]

The origin of eukaryotes represents an enigmatic puzzle, which is still lacking a number of essential pieces. Whereas it is currently accepted that the process of eukaryogenesis involved an interplay between a host cell and an alphaproteo-bacterial endosymbiont, we currently lack detailed information regarding the identity and nature of these players. A number of studies have provided increasing support for the emergence of the eukaryotic host cell from within the archaeal domain of life, displaying a specific affiliation with the archaeal TACK superphylum. Recent studies have shown that genomic exploration of yet-uncultivated archaea, the so-called archaeal 'dark matter', is able to provide unprecedented insights into the process of eukaryogenesis. Here, we provide an overview of state-of-the-art cultivation-independent approaches, and demonstrate how these methods were used to obtain draft genome sequences of several novel members of the TACK superphylum, including Lokiarchaeum, two representatives of the Miscellaneous Crenarchaeotal Group (Bathyarchaeota), and a Korarchaeum-related lineage. The maturation of cultivation-independent genomics approaches, as well as future developments in next-generation sequencing technologies, will revolutionize our current view of microbial evolution and diversity, and provide profound new insights into the early evolution of life, including the enigmatic origin of the eukaryotic cell.

Place, publisher, year, edition, pages
2015. Vol. 370, no 1678, 20140328
Keyword [en]
Archaea, eukaryogenesis, metagenomics, microbial diversity, single-cell genomics, tree of life
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-262947DOI: 10.1098/rstb.2014.0328ISI: 000360552800008OAI: oai:DiVA.org:uu-262947DiVA: diva2:858419
Funder
Swedish Research Council, 621-2009-4813EU, European Research Council, 310039-PUZZLE_CELLSwedish Foundation for Strategic Research , FFL12-0024Carl Tryggers foundation Wenner-Gren Foundations
Available from: 2015-10-02 Created: 2015-09-23 Last updated: 2017-12-01Bibliographically approved

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Saw, Jimmy H.Spang, AnjaZaremba-Niedzwiedzka, KatarzynaJuzokaite, LinaGuy, LionelEttema, Thijs J. G.

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Saw, Jimmy H.Spang, AnjaZaremba-Niedzwiedzka, KatarzynaJuzokaite, LinaGuy, LionelEttema, Thijs J. G.
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Molecular EvolutionScience for Life Laboratory, SciLifeLabDepartment of Medical Biochemistry and Microbiology
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Philosophical Transactions of the Royal Society of London. Biological Sciences
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