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Membrane-associated transcription factor peptidase, site-2 protease, antagonizes ABA signaling in Arabidopsis
Fudan Univ, Sch Life Sci, Inst Plant Biol, Collaborat Innovat Ctr Genet & Dev,State Key Lab, Shanghai 200433, Peoples R China..
Fudan Univ, Sch Life Sci, Inst Plant Biol, Collaborat Innovat Ctr Genet & Dev,State Key Lab, Shanghai 200433, Peoples R China..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Physiological Botany.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Physiological Botany.
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2015 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 208, no 1, 188-197 p.Article in journal (Refereed) Published
Abstract [en]

Abscisic acid plays important roles in maintaining seed dormancy while gibberellins (GA) and other phytohormones antagonize ABA to promote germination. However, how ABA signaling is desensitized during the transition from dormancy to germination is still poorly understood. We functionally characterized the role of membrane-associated transcription factor peptidase, site-2 protease (S2P), in ABA signaling during seed germination in Arabidopsis. Genetic analysis showed that loss-of-function of S2P conferred high ABA sensitivity during seed germination, and expression of the activated form of membrane-associated transcription factor bZIP17, in which the transmembrane domain and endoplasmic reticulum (ER) lumen-facing C-terminus were deleted, in the S2P mutant rescued its ABA-sensitive phenotype. MYC and green fluorescent protein (GFP)-tagged bZIP17 were processed and translocated from the ER to the nucleus in response to ABA treatment. Furthermore, genes encoding negative regulators of ABA signaling, such as the transcription factor ATHB7 and its target genes HAB1, HAB2, HAI1 and AHG3, were up-regulated in seeds of the wild-type upon ABA treatment; this up-regulation was impaired in seeds of S2P mutants. Our results suggest that S2P desensitizes ABA signaling during seed germination through regulating the activation of the membrane-associated transcription factor bZIP17 and therefore controlling the expression level of genes encoding negative regulators of ABA signaling.

Place, publisher, year, edition, pages
2015. Vol. 208, no 1, 188-197 p.
Keyword [en]
ABA response, Arabidopsis, membrane-associated transcription factor, regulated intramembrane proteolysis, seed germination, site-2 peptidase (S2P)
National Category
Botany
Identifiers
URN: urn:nbn:se:uu:diva-262944DOI: 10.1111/nph.13436ISI: 000360376400023PubMedID: 25919792OAI: oai:DiVA.org:uu-262944DiVA: diva2:858431
Available from: 2015-10-02 Created: 2015-09-23 Last updated: 2017-12-01Bibliographically approved

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Valdes, Ana ElisaEngström, Peter

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