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Calcium Signaling in a Genetically Engineered Human Pancreatic beta-Cell Line
Karolinska Inst, Soder Sjukhuset, Dept Clin Sci & Educ, Stockholm, Sweden..
Karolinska Univ Hosp Solna, Dept Mol Med & Surg, Stockholm, Sweden..
Karolinska Univ Hosp Solna, Dept Mol Med & Surg, Stockholm, Sweden.;Karolinska Univ Hosp Solna, Dept Endocrinol Metab & Diabet, Stockholm, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. Karolinska Inst, Soder Sjukhuset, Dept Clin Sci & Educ, Stockholm, Sweden..
2015 (English)In: Pancreas, ISSN 0885-3177, E-ISSN 1536-4828, Vol. 44, no 5, 773-777 p.Article in journal (Refereed) Published
Abstract [en]

Objectives The use of primary human -cells for studying Ca2+ signaling is limited by the scarcity of human pancreatic islets. Rodent insulinoma cell lines are widely used, but it is difficult to extrapolate results obtained from rodent cells to human. Recently, a genetically engineered human -cell line EndoC-BH1 has been developed. We have examined whether the EndoC-BH1 cells could be used as a model for studying Ca2+ signaling in the -cells. Methods We used microscope-based fluorometry to measure cytoplasmic-free Ca2+ concentration from fura-2-loaded single EndoC-BH1 cells cultured on glass cover slips. Ca2+ responses to different agonists of insulin secretion were studied. Insulin secretion was measured by radioimmunoassay. Results EndoC-BH1 cells secreted insulin in response to glucose in a dose-dependent manner, and the secretion was enhanced by GLP-1 (glucagon-like peptide 1). Glucose, potassium chloride, carbachol, l-arginine, and tolbutamide increased cytoplasmic-free Ca2+ concentration in the EndoC-BH1 cells. We found that GLP-1 was essential for Ca2+ response to glucose and tolbutamide. Conclusions We concluded that the EndoC-BH1 cells can be used as model cells to study Ca2+ signaling and stimulus-secretion coupling in the human -cells.

Place, publisher, year, edition, pages
2015. Vol. 44, no 5, 773-777 p.
Keyword [en]
calcium signaling, human -cell line, insulin, stimulus-secretion coupling, EndoC-BH1 cells, [Ca2+](i) - cytoplasmic-free Ca2+ concentration, GLP-1-glucagon-like peptide 1, K-ATP - ATP-sensitive potassium channels, VGCCs - voltage-gated Ca2+ channels
National Category
Gastroenterology and Hepatology
URN: urn:nbn:se:uu:diva-263536DOI: 10.1097/MPA.0000000000000318ISI: 000360629300013PubMedID: 25822155OAI: oai:DiVA.org:uu-263536DiVA: diva2:858854
Available from: 2015-10-05 Created: 2015-10-02 Last updated: 2015-10-05Bibliographically approved

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