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Cotranslational Protein Folding inside the Ribosome Exit Tunnel
Stockholm Univ, Ctr Biomembrane Res, Dept Biochem & Biophys, S-10691 Stockholm, Sweden..
Stockholm Univ, Ctr Biomembrane Res, Dept Biochem & Biophys, S-10691 Stockholm, Sweden..
Univ Munich, CiPS M, Gene Ctr, D-81377 Munich, Germany.;Univ Munich, CiPS M, Ctr Integrated Prot Sci Munich, D-81377 Munich, Germany..
Univ Munich, CiPS M, Gene Ctr, D-81377 Munich, Germany.;Univ Munich, CiPS M, Ctr Integrated Prot Sci Munich, D-81377 Munich, Germany..
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2015 (English)In: Cell reports, ISSN 2211-1247, E-ISSN 2211-1247, Vol. 12, no 10, 1533-1540 p.Article in journal (Refereed) Published
Abstract [en]

At what point during translation do proteins fold? It is well established that proteins can fold cotranslationally outside the ribosome exit tunnel, whereas studies of folding inside the exit tunnel have so far detected only the formation of helical secondary structure and collapsed or partially structured folding intermediates. Here, using a combination of co-translational nascent chain force measurements, inter-subunit fluorescence resonance energy transfer studies on single translating ribosomes, molecular dynamics simulations, and cryoelectron microscopy, we show that a small zinc-finger domain protein can fold deep inside the vestibule of the ribosome exit tunnel. Thus, for small protein domains, the ribosome itself can provide the kind of sheltered folding environment that chaperones provide for larger proteins.

Place, publisher, year, edition, pages
2015. Vol. 12, no 10, 1533-1540 p.
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-264053DOI: 10.1016/j.celrep.2015.07.065ISI: 000360965500002PubMedID: 26321634OAI: oai:DiVA.org:uu-264053DiVA: diva2:859030
Funder
Swedish Foundation for Strategic Research EU, European Research Council, ERC-2008-AdG 232648Swedish Research CouncilKnut and Alice Wallenberg FoundationNIH (National Institute of Health)Wenner-Gren Foundations
Available from: 2015-10-05 Created: 2015-10-05 Last updated: 2017-12-01Bibliographically approved

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