Use of a proximity extension assay proteomics chip to discover new biomarkers for human atherosclerosis
2015 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 242, no 1, 205-210 p.Article in journal (Refereed) Published
Background and aims: We used a proteomics array to simultaneously measure multiple proteins that have been suggested to be associated with atherosclerosis and related them to plaque prevalence in carotid arteries in a human population-based study. Methods: In the Prospective Study of the Vasculature in Uppsala Seniors (PIVUS; n = 931, 50% women, all aged 70 years), the number of carotid arteries with plaques was recorded by ultrasound. Levels of 82 proteins were assessed in plasma by a proximity extension assay (Proseek Multiplex CVD, Olink Bioscience, Uppsala, Sweden) and related to carotid measures in a regression framework. Results: Following adjustment for multiple testing with Bonferroni correction, seven of the proteins were significantly related to the number of carotid arteries affected by plaques in sex-adjusted models (osteoprotegrin, T-cell immunoglobulin and mucin domain (TIM)-1, growth/differentiation factor 15 (GDF-15), matrix metalloprotease-12 (MMP-12), renin, tumor necrosis factor ligand superfamily member 14 (TNFSF14) and growth hormone). Of these, renin (odds ratio [OR], 1.30; 95% confidence interval [CI], 1.13-1.49 per standard deviation increase), growth hormone (OR, 1.24; 95% CI, 1.08-1.43), osteoprotegerin (OR, 1.22; 95% CI, 1.05-1.43) and TNFSF14 (OR, 1.17; 95% CI, 1.01-1.35) were related to plaque prevalence independently of each other and traditional cardiovascular risk factors. Conclusion: A novel targeted proteomics approach using the proximity extension technique discovered several new associations of candidate proteins with carotid artery plaque prevalence in a large human sample.
Place, publisher, year, edition, pages
2015. Vol. 242, no 1, 205-210 p.
Atherosclerosis, Epidemiology, Carotid artery, Ultrasound, Proteomics
Cardiac and Cardiovascular Systems
IdentifiersURN: urn:nbn:se:uu:diva-263438DOI: 10.1016/j.atherosclerosis.2015.07.023ISI: 000360100900032PubMedID: 26204497OAI: oai:DiVA.org:uu-263438DiVA: diva2:859451
FunderKnut and Alice Wallenberg FoundationMarianne and Marcus Wallenberg FoundationEU, European Research Council, 335395-CardiomicsSwedish Heart Lung Foundation, 20120197 20120169Swedish Research Council, 2012-1397 2012-2215Science for Life Laboratory - a national resource center for high-throughput molecular bioscience