Nanocellulose from green algae modulates the in vitro inflammatory response of monocytes/macrophages
2015 (English)In: Cellulose (London), ISSN 0969-0239, E-ISSN 1572-882X, Vol. 22, no 6, 3673-3688 p.Article in journal (Refereed) Published
The response of monocytes and macrophages to functionalized Cladophora nanocellulose (CC) films was evaluated. Carboxyl-CC and hydroxypropyltrimethylammonium-CC [referred to as anionic-CC (a-CC) and cationic-CC (c-CC), respectively] were synthesized by TEMPO-mediated oxidation and epoxypropyltrimethylammonium chloride condensation of unmodified CC (u-CC). The cell response to u-CC, a-CC and c-CC of untreated and phorbol 12-myristate-13 acetate treated THP-1 cells, i.e. monocytes and macrophages, in the presence and absence of lipopolysaccharide (LPS) was studied. u-CC impairs the viability of THP-1 monocytes and macrophages most probably due to the presence of impurities. In the absence of LPS, the functionalized materials behave as inert materials in terms of the inflammatory response of both monocytes and differentiated macrophages. Under pro-inflammatory stimuli the functionalized CC films suppressed the inflammatory response induced by LPS. The a-CC material with its aggregated, aligned fibre structure caused a more pronounced reduction of TNF-alpha levels compared to the c-CC film that exhibited non-aggregated, randomly oriented fibres. These results push forward the option of using functionalized CC materials in the biomedical field.
Place, publisher, year, edition, pages
2015. Vol. 22, no 6, 3673-3688 p.
Cladophora nanocellulose, Biocompatibility, Inflammation, Monocytes/macrophages
Research subject Engineering Science with specialization in Nanotechnology and Functional Materials
IdentifiersURN: urn:nbn:se:uu:diva-264383DOI: 10.1007/s10570-015-0772-2ISI: 000364513800014OAI: oai:DiVA.org:uu-264383DiVA: diva2:860089
FunderSwedish Research Council FormasKnut and Alice Wallenberg Foundation