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Middle ear mucosa changes following exposure to Pseudomonas aeruginosa exotoxin A
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences. (Ear-Nose-Throat)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences. (Ear-Nose-Throat)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences. (Ear-Nose-Throat)
1999 (English)In: European Archives of Oto-Rhino-Laryngology, ISSN 0937-4477, E-ISSN 1434-4726, Vol. 256, no 10, 484-90 p.Article in journal (Refereed) Published
Abstract [en]

Pseudomonas aeruginosa exotoxin A (1 microgram/20 microliters) was instilled into the middle ear cavity in the rat. Morphological changes of the mucosa were analyzed after various intervals using light (LM) and transmission electron microscopy (TEM). An inflammatory cell reaction was seen in which there was a predominance of polymorphonuclear leukocytes (PMNs) and mononuclear cells that were mostly lymphocytes and monocytes/macrophages. Epithelial cells often showed signs of metaplasia and hyperplasia, followed by degradation through necrosis and/or apoptosis, resulting in denudation of the submucosal layer. A characteristic feature was the physical interaction between intraepithelial lymphocytes and epithelial cells. Lymphocytes adhering to the cell coat of epithelial cells showed signs of directed secretion that seemed to end in necrosis or apoptosis of the target cell. These changes occurred simultaneously with phagocytosis of cells and cell debris by PMNs and macrophages. Ultrastructural analysis suggested that intraepithelial, lymphocyte-mediated cytolysis and apoptosis may be important events in degradation of the epithelium following exposure to Pseudomonas sp. exotoxin. These findings indicate that denuding of the lamina propria may facilitate the penetration of toxin into the labyrinth, thus explaining subsequent inner ear damage.

Place, publisher, year, edition, pages
1999. Vol. 256, no 10, 484-90 p.
Keyword [en]
Pseudomonas aeruginosa exotoxin, Ultrastructural changes, Inflammatory cell reactions, Middle ear mucosa
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-58115DOI: 10.1007/s004050050196PubMedID: 10638354OAI: oai:DiVA.org:uu-58115DiVA: diva2:86024
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2017-12-01Bibliographically approved

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