Image analysis of neurofilament immunoreactivity in human spiral ganglion
1997 (English)In: Journal for Oto-Rhino-Laryngology, ISSN 0301-1569, Vol. 59, no 2, 258-62 p.Article in journal (Refereed) Published
Computer-assisted image analysis was used to study the immunoreactivity to NF-L, NF-M and NF-H in human spiral ganglion cells. The concentrations, represented by the relative mean grey of NF-L, NF-M and NF-H, were calculated and compared. The cell-area mean-grey correlations for NF-L, NF-M and NF-H were analysed and calculated, showing that NF-M is more specific to the larger cells (type 1?) and NF-H is more specific for the smaller cells (type 2?), while NF-L is non-specific for cell size. These findings confirm several previous assumptions by providing a quantitative basis. We conclude that image analysis is a useful-even essential-tool for the analysis of immunostained temporal bone sections.
Place, publisher, year, edition, pages
1997. Vol. 59, no 2, 258-62 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-58165PubMedID: 9279863OAI: oai:DiVA.org:uu-58165DiVA: diva2:86074