uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines
Show others and affiliations
2015 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 144, 1030-1035 p.Article in journal (Refereed) Published
Abstract [en]

Current methods for the identification and/or quantification of viral proteins in influenza virus and virosome samples suffer from long analysis times, limited protein coverage and/or low accuracy and precision. We studied and optimized capillary gel electrophoresis (CGE) in order to achieve faster and enhanced characterization and quantification of viral proteins. Sample preparation as well the composition of the gel buffer was investigated in order to achieve adequate protein separation in relatively short times. The total sample preparation (reduction and deglycosylation) could be carried out efficiently within two hours. Hydrodynamic injection, separation voltage, and capillary temperature were optimized in full factorial design. The final method was validated and showed good performance for hemagglutinin fragment 1 (HA1), hemagglutinin fragment 2 (HA2), matrix protein (M) and nucleoprotein (NP). The CGE method allowed identification of different virus strains based on their specific protein profile. B/Brisbane inactivated virus and virosome samples could be analyzed within one day. The CGE results (titers) were comparable to single radial immune-diffusion (SRID), but the method has the advantage of a much faster time to results. CGE analysis of A/Christchurch from upstream process demonstrated the applicability of the method to samples of high complexity. The CGE method could be used in the same analyte concentration range as the RP-HPLC method, but showed better precision and accuracy. Overall, the total analysis time for the CGE method was much shorter, allowing analysis of 100 samples in 4 days instead of 10 days for SRID.

Place, publisher, year, edition, pages
2015. Vol. 144, 1030-1035 p.
Keyword [en]
Capillary gel electrophoresis; Influenza; Viral proteins; Hemagglutinin; Quantification; CESDS
National Category
Pharmaceutical Sciences Analytical Chemistry
URN: urn:nbn:se:uu:diva-264579DOI: 10.1016/j.talanta.2015.07.047ISI: 000363346200140PubMedID: 26452923OAI: oai:DiVA.org:uu-264579DiVA: diva2:861025
Available from: 2015-10-15 Created: 2015-10-15 Last updated: 2015-11-27Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Analytical Pharmaceutical Chemistry
In the same journal
Talanta: The International Journal of Pure and Applied Analytical Chemistry
Pharmaceutical SciencesAnalytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 116 hits
ReferencesLink to record
Permanent link

Direct link