uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 77, 32-39 p.Article in journal (Refereed) Published
Abstract [en]

A turn-on competitive immunoassay using a low-cost Blu-ray optomagnetic setup and two differently sized magnetic particles (micron-sized particles acting as capture particles and nano-sized particles acting as detection particles) is here presented. For Salmonella detection, a limit of detection of 8 x 10(4) CFU/mL is achieved within a total assay time of 3 h. The combination of a competitive strategy and an optomagnetic setup not only enables a turn-on read-out format, but also results in a sensitivity limit about a factor of 20 times lower than of volumetric magnetic stray field detection device based immunoassays. The improvement of sensitivity is enabled by the formation of immuno-magnetic aggregates providing steric hindrance protecting the interior binding sites from interaction with the magnetic nanoparticle labels. The formation of immuno-magnetic aggregates is confirmed by fluorescence microscopy. The system exhibits no visible cross-reaction with other common pathogenic bacteria, even at concentrations as high as 10 CFU/mL. Furthermore, we present results when using the setup for a qualitative and homogeneous biplex immunoassay of Escherichia coli and Salmonella typhimurium.

Place, publisher, year, edition, pages
2016. Vol. 77, 32-39 p.
Keyword [en]
Biosensors; Immunoassays; Magnetic particles; Magneto-optics
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
URN: urn:nbn:se:uu:diva-264720DOI: 10.1016/j.bios.2015.08.070ISI: 000366766900006OAI: oai:DiVA.org:uu-264720DiVA: diva2:861185
Funder
Swedish Research Council Formas, 221-2012-444
Available from: 2015-10-15 Created: 2015-10-15 Last updated: 2017-12-01Bibliographically approved

Open Access in DiVA

fulltext(1942 kB)220 downloads
File information
File name FULLTEXT01.pdfFile size 1942 kBChecksum SHA-512
54413366dbc03f330a1c486ea45d50f6bf51101ecc4095eba90256e4f0df63396322c2196d120f006a8da42a9ef39c9d40e1ec888fccd39be634c1e5dc7f7954
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Tian, BoStjernberg Bejhed, RebeccaSvedlindh, PeterStrömberg, Mattias

Search in DiVA

By author/editor
Tian, BoStjernberg Bejhed, RebeccaSvedlindh, PeterStrömberg, Mattias
By organisation
Solid State Physics
In the same journal
Biosensors & bioelectronics
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 220 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 815 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf