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Exogenous plant hormones and cyclotide expression in Viola uliginosa (Violaceae)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy. Jagiellonian Univ, Inst Bot, Dept Plant Cytol & Embryol, PL-30387 Krakow, Poland.;Polish Acad Sci, W Szafer Ist Bot, PL-31512 Krakow, Poland..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
Jagiellonian Univ, Inst Bot, Dept Plant Cytol & Embryol, PL-30387 Krakow, Poland..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy. (Ulf Goransson)
2015 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 117, 527-536 p.Article in journal (Refereed) Published
Abstract [en]

Plants from Violaceae produce cyclotides, peptides characterized by a circular peptide backbone and a cystine knot. This signature motif gives stability that can harness a wide spectrum of biological activities, with implications in plant defense and with applications in medicine and biotechnology. In the current work, cyclotide expressing in vitro cultures were established from Viola uliginosa. These cultures are useful models for studying biosynthesis of cyclotides and can also be used in their production. The cyclotide expression pattern is shown to be dependent on exogenous plant growth regulators, both on peptide and gene expression levels. The highest yields of cyclotides were obtained on media containing only a cytokinin and were correlated with storage material accumulation. Exposure to auxins decreased cyclotide production and caused shifting of the biosynthesis pattern to root specific cyclotides. The response to stimuli in terms of cyclotide expression pattern appears to be developmental, and related to polar auxin transportation and the auxin/cytokinin ratio regulating tissue differentiation. By the use of whole transcriptome shotgun sequencing (WTSS) and peptidomics, 20 cyclotide sequences from V. uliginosa (including 12 new) and 12 complete precursor proteins could be identified. The most abundant cyclotides were cycloviolacin O3 (CyO3), CyO8 and CyO13. A suspension culture was obtained that grew exponentially with a doubling time of approximately 3 days. After ten days of growth, the culture provided a yield of more than 4 mg CyO13 per gram dry mass.

Place, publisher, year, edition, pages
2015. Vol. 117, 527-536 p.
Keyword [en]
Viola uliginosa (Violaceae), Cyclotides, In vitro culture, Plant growth regulators, Whole transcriptome shotgun sequencing, Mass spectrometry
National Category
Botany Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-264668DOI: 10.1016/j.phytochem.2015.07.016ISI: 000361253300055PubMedID: 26246035OAI: oai:DiVA.org:uu-264668DiVA: diva2:861338
Funder
Swedish Research Council, 621-2007-5167Swedish Foundation for Strategic Research , F06-0058
Available from: 2015-10-16 Created: 2015-10-15 Last updated: 2017-12-01

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Jacobsson, ErikGoransson, Ulf

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