uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
In situ molecular profilling of the microenvironment of breast carcinoma
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2015 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

High stromal PDGF receptor B expression was shown to have strong prognostic value in a studyinvolving over 600 breast cancer patients however, the molecular role of the receptor in tumordevelopment remains unclear. In this project we studied the spatial distribution and expressionlevels of a panel genes and markers associated with PDGF signaling, in breast cancer tumormicroenvironment (TME) using a newly developed technique -in situ sequencing. The techniquerelies on padlock probes which we validated with corresponding RNA sequencing, microarray,and immunohistochemistry data. Our results showed that high PDGF receptor B mRNA colocalizedwith markers of two pathways, TGFβ and Hedgehog signaling; this suggests that theymight contribute to the PDGF-receptor B-driven tumor growth. We also showed that stromalPDGF signaling is stimulated predominantly by tumor cells. Finally, further expression profilingof each individual gene revealed that CXCL14 was mainly expressed in the stroma, ACTA2expression was enriched in the tumor/stroma boundary while the stem-cell marker, OCT3, wasexpressed in the interior of the tumor cells.

Place, publisher, year, edition, pages
2015. , 34 p.
Keyword [en]
Sequencing, gene expression analysis, cancer genetics, RCA, padlock probe
National Category
Cell and Molecular Biology
URN: urn:nbn:se:uu:diva-265258OAI: oai:DiVA.org:uu-265258DiVA: diva2:864241
Educational program
Master Programme in Biology
2015-06-03, B7:113a, Biomedical Centre, Uppsala, 13:00 (English)
Available from: 2016-09-14 Created: 2015-10-26 Last updated: 2016-09-14Bibliographically approved

Open Access in DiVA

fulltext(2129 kB)8 downloads
File information
File name FULLTEXT01.pdfFile size 2129 kBChecksum SHA-512
Type fulltextMimetype application/pdf

By organisation
Biology Education Centre
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 8 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 2 hits
ReferencesLink to record
Permanent link

Direct link