uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
On the mechanism of translation error induction by aminoglycoside antibiotics
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

We used a cell-free system with pure Escherichia coli components to study how the aminoglycosides disrupt initial codon selection of aminoacyl-tRNAs in ternary complex with elongation factor Tu and GTP by messenger RNA-programmed ribosomes. We take the advantage of the universal rate-accuracy trade-off for all enzymatic selections to determine how the efficiency of initial codon readings decreased linearly toward zero as the accuracy of discrimination against near cognate and wobble codon readings increased toward the maximal asymptote, the effective d value. It turns out two ribosome bound states of ternary complex. First, one codon non-selective step, Mg2+ ions shift the equilibrium from free to ribosome bound ternary complex meaning that the equilibrium between the two ribosome bound complexes is unaltered. Second, codon selective step, aminoglycosides shift the equilibrium from codon non-selective to codon selective complex meaning that the equilibrium between the free and the codon non-selective state is unchanged.

Keyword [en]
protein synthesis, genetic code, misreading, kinitics, aminoglycosides, decoding
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-265304OAI: oai:DiVA.org:uu-265304DiVA: diva2:865097
Available from: 2015-10-26 Created: 2015-10-26 Last updated: 2015-10-26

Open Access in DiVA

No full text

Authority records BETA

Zhang, Jingji

Search in DiVA

By author/editor
Zhang, Jingji
By organisation
Structure and Molecular Biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 270 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf