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Highly repeatable synthesis of nHA with high aspect ratio
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences. (materials in medicine)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Univ Oslo, Dept Phys, Oslo, Norway; Simon Fraser Univ, Dept Chem, Burnaby, BC V5A 1S6, Canada.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
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2015 (English)In: Materials letters (General ed.), ISSN 0167-577X, E-ISSN 1873-4979, Vol. 159, 163-167 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2015. Vol. 159, 163-167 p.
National Category
Biomaterials Science Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-265712DOI: 10.1016/j.matlet.2015.06.086ISI: 000362603000043OAI: oai:DiVA.org:uu-265712DiVA: diva2:866437
Funder
Swedish Research Council, 2013-5419VINNOVA, 2010-01907EU, FP7, Seventh Framework Programme, INFRA-2010-262163
Available from: 2015-11-02 Created: 2015-11-02 Last updated: 2017-12-01
In thesis
1. Nanofeatures of Biomaterials and their Impact on the Inflammatory Response
Open this publication in new window or tab >>Nanofeatures of Biomaterials and their Impact on the Inflammatory Response
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Nanomaterials offer an advantage over traditional biomaterials since cells naturally communicate via nanoscale interactions. The extracellular matrix, for example, modulates adhesion and cellular functions via nanoscale features. Thus incorporating nanofeatures into biomaterials may promote tissue regeneration, however in certain forms and doses nanomaterials can also cause harm. A thorough understanding of cell-nanomaterial interactions is therefore necessary to better design functional biomaterials. This thesis focuses on evaluating the effect of nanofeatures on inflammation using two different models: nanoporous alumina and hydroxyapatite nanoparticles (HANPs).

The inflammatory response caused by in vitro exposure of macrophages to nanoporous alumina, with pore diameters of 20nm and 200nm, was investigated. In addition in vivo studies were performed by implantation of nanoporous membranes in mice. In both cases the 200nm pore diameter elicited a stronger inflammatory response.

Nanoporous alumina with 20, 100 and 200nm pores were loaded with Trolox, a vitamin E analogue, in order to scavenge ROS produced by primary human polymorphonuclear (PMNC) and mononuclear (MNCs) leukocytes. Unloaded alumina membranes stimulated greater ROS production from PMNCs cultured on 20nm versus 100nm pores. This trend reversed when PMNCs were cultured on Trolox loaded membranes since Trolox eluted slower from 20nm than 100nm and 200nm pores. ROS produced from PMNCs was reduced between 8-30% when cultured on Trolox loaded membranes. For MNCs, ROS production was not affected by pore size. However when the alumina was loaded with Trolox ROS production was quenched by 95%.

HANPs with distinct morphologies (long rods, sheets, dots, and fibers) were synthesized via hydrothermal and precipitation methods. The HANPs were then exposed to PMNCs, MNCs, and the human dermal fibroblast (hDF) cell line. Changes in cell viability, ROS, morphology, and apoptotic behavior were evaluated. PMNC and hDF viability decreased following exposure to fibers, while the dot particles reduced MNC viability. Fibers stimulated greater ROS production from PMNCs and MNCs, and caused apoptotic behavior in all cell types. Furthermore, they also stimulated greater capsule thickness in vivo, suggesting that nanoparticle morphology can significantly influence acute inflammation.

The outcome of this thesis, confirms the importance of understanding how nanofeatures influence inflammation.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2016. 70 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1374
Keyword
Nanofeatures, alumina, hydroxyapatite, inflammation
National Category
Nano Technology Biological Sciences Materials Engineering
Research subject
Engineering Science with specialization in Materials Science
Identifiers
urn:nbn:se:uu:diva-284402 (URN)978-91-554-9576-3 (ISBN)
Public defence
2016-06-09, Å2001, Lägerhyddsvägen 1, Uppsala, 09:30 (English)
Opponent
Supervisors
Available from: 2016-05-19 Created: 2016-04-18 Last updated: 2016-06-01
2. Glass Ionomer Cements with Improved Bioactive and Antibacterial Properties
Open this publication in new window or tab >>Glass Ionomer Cements with Improved Bioactive and Antibacterial Properties
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Dental restorative cements are placed in a harsh oral environment where they are subjected to thermal shock, chemical degradation, and repeating masticatory force. The ideal restorative dental cements should have superior mechanical properties, chemical stability, aesthetic, good handling properties, biocompatibility, antibacterial properties, and preferably bioactivity. This thesis presents research on dental restorative cements with enhanced properties. The overall aim was to increase the bioactivity and antibacterial properties of dental restorative cements without affecting their other properties.

The effect from adding calcium silicate to glass ionomer cement (GIC) was investigated. The results showed that calcium silicate could increase the bioactivity and reduce the cytotoxicity of conventional glass ionomer cement without compromising its setting and mechanical properties.

Hydroxyapatite (HA) with a high aspect ratio and thin nacreous-layered monetite sheets were also synthesized. Nano HA particles with an aspect ratio of 50 can be synthesized by both precipitation and hydrothermal methods. The aspect ratio was controlled via the pH of reaction medium. Thin nacreous-layered monetite sheets were synthesized through a self-assembly process in the presence of an amine based cationic quaternary surfactant. Temperature, pH, and presence of surfactant played essential roles in forming the nacreous-layered monetite sheets. Then the effect from adding silver doped HA and monetite particles was investigated. The results showed that the antibacterial properties of GIC could be increased by incorporating silver doped HA and monetite particles. Further examination showed that the pH change, F- ion release, and concentration of released Ag+ ions were not responsible for the improved antibacterial properties.

The quasi-static strengths and compressive fatigue limits of four types of the most commonly used dental restorations were evaluated. In our study, resin modified GIC and resin-based composite showed superior static compressive strength and fatigue limits compared to conventional GIC. The static compressive strength of dental cements increased with the aging time. However, aging had no effect on the compressive fatigue limit of resin modified GIC and resin-based composite. The compressive fatigue limit of conventional GIC even showed a drastic decrease after aging.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2016. 62 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1413
Keyword
biomaterial, glass ionomer cement, bioactivity, hydroxyapatite, monetite, calcium silicate
National Category
Biomaterials Science
Identifiers
urn:nbn:se:uu:diva-301924 (URN)978-91-554-9670-8 (ISBN)
Public defence
2016-10-14, Å2005, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2016-09-21 Created: 2016-08-25 Last updated: 2016-09-22

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Chen, SongPujari-Palmer, ShiuliRubino, StefanoWestlund, ViktoriaOtt, MarjamEngqvist, HåkanXia, Wei

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