Effect of Pseudomonas aeruginosa exotoxin A on inner ear function
1997 (English)In: Acta Oto-Laryngologica, ISSN 0001-6489, Vol. 117, no 1, 73-79 p.Article in journal (Refereed) Published
Electrophysiological changes were studied in the albino rat following instillation of Pseudomonas aeruginosa exotoxin A into the middle ear cavity through the tympanic membrane. Hearing threshold was measured by a burst-elicited, frequency-specific auditory brainstem response (ABR) technique prior to exposure, then 24 and 48 h, 5 days, 2 and 4 weeks after the toxin instillation. A single dose (1 microgram/20 microliters) of Pseudomonas aeruginosa exotoxin A raised the ABR threshold over the whole frequency range, by 5-25 dB, particularly in the high tones. All threshold shifts were of combined conductive and cochlear type, reversible, with deterioration starting at 24-48 h and recovery at 2-4 weeks. Effusion of serous fluid occurred at 24 or 48 h, resulting in conductive hearing loss. Latency/intensity curves revealed a cochlear component in addition to conductive hearing loss. Morphological examination by SEM showed slight and inconsistent derangement of OHCs. It is concluded that Pseudomonas aeruginosa exotoxin A causes middle ear inflammation, facilitating penetration to the inner ear and that this toxin also reversibly affects cochlear function.
Place, publisher, year, edition, pages
1997. Vol. 117, no 1, 73-79 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-58740PubMedID: 9039485OAI: oai:DiVA.org:uu-58740DiVA: diva2:86649