In Vivo Effects of Mesenchymal Stromal Cells in Two Patients With Severe Acute Respiratory Distress Syndrome
2015 (English)In: Stem Cells Translational Medicine, ISSN 2157-6564, E-ISSN 2157-6580, Vol. 4, no 10, 1199-1213 p.Article in journal (Refereed) Published
Mesenchymal stromal cells (MSCs) have been investigated as a treatment for various inflammatory diseases because of their immunomodulatory and reparative properties. However, many basic questions concerning their mechanisms of action after systemic infusion remain unanswered. We performed a detailed analysis of the immunomodulatory properties and proteomic profile of MSCs systemically administered to two patients with severe refractory acute respiratory distress syndrome (ARDS) on a compassionate use basis and attempted to correlate these with in vivo anti-inflammatory actions. Both patients received 2 x 10(6) cells per kilogram, and each subsequently improved with resolution of respiratory, hemodynamic, and multiorgan failure. In parallel, a decrease was seen in multiple pulmonary and systemic markers of inflammation, including epithelial apoptosis, alveolar-capillary fluid leakage, and proinflammatory cytokines, microRNAs, and chemokines. In vitro studies of the MSCs demonstrated a broad anti-inflammatory capacity, including suppression of T-cell responses and induction of regulatory phenotypes in T cells, monocytes, and neutrophils. Some of these in vitro potency assessments correlated with, and were relevant to, the observed in vivo actions. These experiences highlight both the mechanistic information that can be gained from clinical experience and the value of correlating in vitro potency assessments with clinical effects. The findings also suggest, but do not prove, a beneficial effect of lung protective strategies using adoptively transferred MSCs in ARDS. Appropriate randomized clinical trials are required to further assess any potential clinical efficacy and investigate the effects on in vivo inflammation. STEM CELLS TRANSLATIONAL MEDICINE 2015;4:1199-1213
Place, publisher, year, edition, pages
2015. Vol. 4, no 10, 1199-1213 p.
Acute respiratory distress syndrome, Pulmonary diseases, Respiratory tract, Stem cells, Cell transplantation, Bone marrow stromal cells, Cellular therapy, Clinical translation
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:uu:diva-265697DOI: 10.5966/sctm.2015-0021ISI: 000362151800014PubMedID: 26285659OAI: oai:DiVA.org:uu-265697DiVA: diva2:866685
FunderSwedish Childhood Cancer FoundationThe Karolinska Institutet's Research FoundationStockholm County CouncilSwedish Cancer SocietySwedish Research CouncilVINNOVASwedish Heart Lung FoundationSwedish Society for Medical Research (SSMF)
De 2 första författarna delar förstaförfattarskapet.
De 3 sista författarna delar sistaförfattarskapet.2015-11-032015-11-022015-12-29Bibliographically approved