uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Programmed cell death in the development of the mouse external auditory canal
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences. (Oto-Rhino-Laryngology, Head & Neck Surgery)
Show others and affiliations
1998 (English)In: Anatomical Record, ISSN 0003-276X, Vol. 252, no 3, 378-82 p.Article in journal (Refereed) Published
Abstract [en]

Programmed cell death (PCD) is an essential event for development. The purpose of this work was to ascertain how PCD, in vivo designated apoptosis, is involved in the development of the external auditory canal. We performed a time sequence study of the distribution of apoptosis during the development of external auditory canal (EAC) of the mouse. ICR mice ranging in age from embryonic day 11.5 (E11.5) to 12 days after birth (DAB) were used in the present study. A part of each head including both ears was removed and was processed according to its purpose. Light and electron microscopy for morphological studies and TUNEL method (Gavrieli et al. [1992] J Cell Biol., 119:493-501) for histochemical studies were used. On E11.5, distinct TUNEL-positive staining occurred in the branchial arch. Between E15.5 and 1DAB, TUNEL-positive cells were observed throughout the EAC and the number of these cells decreased with age. On E15.5 and E16.5, numerous TUNEL-positive cells were observed in a cavity remained in the epithelial plate. Transmission electron microscopy revealed that these cells had the features of apoptosis. From 3-12 DAB, no apoptosis was observed in the EAC except for the terminal differentiation of the skin of the EAC. Apoptosis was not observed during recanalization of the EAC, but occurred during the formation of the epithelial plate. The investigation established that PCD is involved in the formation of the epithelial plate, whereas only cornification of the epithelium of the EAC is associated with recanalization.

Place, publisher, year, edition, pages
1998. Vol. 252, no 3, 378-82 p.
Keyword [en]
programmed cell death, apoptosis, external auditory canal, development, mouse
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-58760DOI: 10.1002/(SICI)1097-0185(199811)252:3<378::AID-AR5>3.0.CO;2-#PubMedID: 9811215OAI: oai:DiVA.org:uu-58760DiVA: diva2:86669
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2010-03-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Surgical Sciences
In the same journal
Anatomical Record
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 171 hits
ReferencesLink to record
Permanent link

Direct link