Chemical modification of Cladophora nanocellulose to provide a non-toxic material with anticoagulant properties
2015 (English)Conference paper, Poster (Refereed)
Blood purification is a common procedure for treating patients with kidney failure in which an extracorporeal device filled with a porous material containing antibodies can retain toxins from the blood. In order to avoid activation and coagulation, heparin is administrated to patients; however this sulfonated polysaccharide may cause many side effects such as intense bleeding and osteoporosis. In this project nanocellulose from Cladophora green algae was used for the development of a porous material with anticoagulant properties. A periodate oxidation followed by an amine cross-linking and subsequent reduction produced dialdehyde cellulose beads ranging from 10-100 mm in diameter with improved mechanical properties and high stability in alkaline media. This material was then sulfonated to acquire anticoagulant properties and characterized by FTIR, z-potential, condutometric titration, elemental analysis and BET surface area showing that its porosity varies with the degree of sulfonation. After extensive washing, toxicology experiments were performed with a THP-1 monocyte cell line in order to examine if the material was non-toxic and could be suitable as a matrix in blood purification.
Place, publisher, year, edition, pages
IdentifiersURN: urn:nbn:se:uu:diva-266842OAI: oai:DiVA.org:uu-266842DiVA: diva2:868895
4th International Polysaccharide Conference (EPNOE 2015), Warsaw, Poland,19-22 October 2015